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Studies On The Cross Reactivity On Vaccinal Strains Of Infectious Bronchitis Virus

by Bushra Zameer | Dr.Khushi Muhammad | Dr.Khalid Saeed | Dr.Masood Rubani | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 2002Dissertation note: Cross reactivity of four different strains of infectious bronchitis virus (D-274, D-1466, 4/91 and H-120) has been studied. All these strains induced stunting growth and increased volume of allanto-amniotic fluid (AAF). Killed oil based vaccine of each strain was prepared and used to produce hyperimmune serum in chickens. The hyperimmune serum was used in cross haemagglutination inhibition (HI) and cross neutralization tests. The results of cross HI showed that D-274 and D-1466 shared 7.48% antigenic similarity with each other and H-120 sharedl3% and 8% antigenic similarities with D-274 and D-1466, respectively. The results of cross Neutralization showed that no cross neutralization present among these virus strains. So vaccine having each strain is required to be used to control the problem. Availability: Items available for loan: UVAS Library [Call number: 0801,T] (1).

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A Study On Bacteriological Examination Of Raw And Pasteurized Milk Marketed In Lahore Area With Particular Reference To Public Health

by Aman Ullan | Dr.Muhammad Akram Munir | Dr.Khalid Saeed | Dr.Masood Rubani | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2001Dissertation note: The present study was conducted to study the bacteriological quality of various types of milk being marketed in Lahore. A total of 120 milk samples consisting of equal number (30 each of raw, pasteurized, UHT and sterilized milk) from various sources were examined for standard plate counts using milk agar, coliform count using EMB agar and using milk ring test (MRT) for brucellosis. All the samples were negative for MRT. Average standard plate count (SPC) and coliform counts for raw milk were 2.34x107 cfulml and 2.2x105 cfu/ml respectively. Similarly, SPC and coliform counts for pasteurized milk were found as 2.16x105 cfuJml and 3.28x104 cfu/ml respectively. No raw milk sample fulfilled the International Standards for very good quality milk for SPC or coliform count. For pasteurized milk, only 33.33% samples fulfilled the International Standards for very good pasteurized milk. SPC and coliform counts for UHT or sterilized milk was zero thus fulfilling the International Standards. A total of 162 isolates (88 isolates from raw milk and 74 isolates from the pasteurized milk) were identified. These isolates included Staphylococcus epidermidis, 38 strain (21 from raw milk and 17 from pasteurized milk), Staph. aureus, 30 strains (16 from raw and 14 from pasteurized milk), Escherichia coli, 24 stains (14 and 10), Enterobacter aerogenes, 20 (11, 09), Bacillus cereus, 22 (15, 07), Micrococcus luteus 15, (03, 12) and Klebsiellapneumoniae 13 (08, 05) strains. vitro antibiotic sensitivity tests indicated that all the isolates were resistant to penicillin whereas most of the isolates were sensitive to Gentamicin, Kanamycin and Chloramphenicol. Escherichia coli was resistant to most of the commonly used antibiotics. Overall hygienic quality of raw and pasteurized milk available in Lahore city was graded as poor. Availability: Items available for loan: UVAS Library [Call number: 0802,T] (1).

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